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Desalting and concentration of proteins in dilute solutions using reversed phase HPLC
Summary
Type:
HPLC
Application #:
108560
Detection:
UV, 230 nm
Conditions:
buffer A: 0.1% trifluoroacetic acid (TFA) in water, buffer B: 0.1% TFA in 2-propanol
Authors:
Pohl T
Source:
Anal Biochem 160 (1987)
Details
Legend: Fig. 1. Chromatogram of the desalting procedure of a ribosomal protein mixture (1mg in 5ml 6M urea).
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